Abstract ↔ claims

run not observed · v1 provisional awaiting approval

Which claims does the abstract carry, and which does it drop?

for Impaired excitability of fast-spiking neurons in a novel mouse model of KCNC1 epileptic encephalopathy · this layer across all papers · json

Provisional

This layer needs a corpus-scope decision that has not been ruled on yet, so what it produces would change if the decision changed. It waits on claim-format, relation-vocab.

Awaiting approval

Waiting for approval. That is a statement about the record, not about whether anyone has read this: people read the corpus without stamping what they read, and only a stamp leaves a trace. Approval is an operation on a version, not a step of its own — it is recorded against the version it was granted to, so running this layer again does not carry it forward.

What this layer produced

Abstract mapped to claims

The paper's abstract is shown with each sentence linked to the claim(s) it represents in the dependency graph. Hover or click a sentence to highlight the corresponding claim cards. Below: what the graph contains that the abstract leaves out, and vice versa.

Abstract

1The recurrent pathogenic variant KCNC1-p.Ala421Val (A421V) is a cause of developmental and epileptic encephalopathy characterized by moderate-to-severe developmental delay/intellectual disability, and infantile-onset treatment-resistant epilepsy with multiple seizure types, including myoclonic seizures. 2Yet, the mechanistic basis of this disease, and of the KCNC1 disease spectrum, remains unclear. 3KCNC1 encodes Kv3.1, a voltage-gated potassium channel subunit that is strongly and selectively expressed in neurons capable of generating action potentials at high frequency, including parvalbumin-positive fast-spiking GABAergic inhibitory interneurons in cerebral cortex (PV-INs) that are known to be important for cognitive function and plasticity as well as control of network excitation to prevent seizures. 4In this study, we generate a novel transgenic mouse model with conditional expression of the A421V pathogenic missense variant (Kcnc1-A421V/+ mice) to explore the specific physiological mechanisms of KCNC1 developmental and epileptic encephalopathy. 5Our results indicate that global heterozygous expression of the A421V variant leads to cognitive impairment, epilepsy, and premature lethality. 6We observe decreased PV-IN cell surface expression of Kv3.1 via immunohistochemistry, decreased voltage-gated potassium current density in PV-INs using outside-out nucleated macropatch recordings in brain slice, and profound impairments in the intrinsic excitability of cerebral cortex PV-INs (but not excitatory neurons) via current-clamp electrophysiology. 7In vivo two-photon calcium imaging revealed altered activity in Kcnc1-A421V/+ PV-INs and excitatory cells, as well as hypersynchronous discharges correlated with brief paroxysmal movements that were subsequently shown to be myoclonic seizures on electroencephalography. 8We found alterations in PV-IN-mediated inhibitory neurotransmission in young adult but not juvenile Kcnc1-A421V/+ mice relative to wild-type controls. 9Together, these results establish the specific impact of the recurrent Kv3.1-A421V variant on neuronal excitability and synaptic physiology across development to drive network dysfunction underlying KCNC1 epileptic encephalopathy.

[1]
no corresponding claim in the graph
[2]
no corresponding claim in the graph
[3]
no corresponding claim in the graph
[4]
no corresponding claim in the graph
[5]
synthesis across claims → H2.P1.1 · Young adult Kcnc1-A421V/+ mice (P35–65) exhibit significantly longer escape late, H2.P4.1 · 8 of 12 Kcnc1-A421V/+ mice exhibit convulsive spontaneous seizures on video-EEG , E1 · All Kcnc1-A421V/+ knock-in mice die before 122 days of age, while wild-type litt, H2.P1 · Kcnc1-A421V/+ mice should show specific cognitive deficits in spatial learning (, H2.P4 · Kcnc1-A421V/+ mice should show spontaneous convulsive seizures, SUDEP events, an, H2 · Cell-autonomous Kv3.1 LOF in PV-INs is sufficient to drive the KCNC1 DEE phenoty
[6]
direct map → H1.P1.1 · The ratio of membrane to cytosolic Kv3.1 immunofluorescence intensity is signifi, H1.P2.1 · Parvalbumin-positive interneurons (PV-INs) in Kcnc1-A421V/+ mice show significan, H3.P3.2 · PV-INs from Kcnc1-A421V/+ mice exhibit impaired maximal firing frequency in patc, H3.P3.1 · PV-INs from Kcnc1-A421V/+ mice show significantly reduced AP downstroke velocity, H3.P1.2 · No significant differences in synaptic transmission or intrinsic excitability ar, H3.P1.1 · Excitatory neurons from adult (P32–42) Kcnc1-A421V/+ mice show no significant di, H1.P1 · If A421V is a trafficking lesion, mutant PV-INs should show reduced membrane:cyt, H1.P2 · Heterozygous Kcnc1-A421V/+ PV-INs should show reduced voltage-gated K+ current d, H3.P3 · Kcnc1-A421V/+ PV-INs should show reduced maximal firing, slower AP downstroke, a, H3.P1 · Excitatory neurons should show no significant impairment of K+ current, firing, , H1 · KCNC1-A421V is a Kv3.1 loss-of-function variant whose primary lesion is impaired, H3 · Kv3.1 loss-of-function should selectively impair fast-spiking PV-INs that depend
[7]
direct map → H2.P2.2 · During quiet rest, PV– (excitatory) cells in Kcnc1-A421V/+ mice show significant, H2.P2.1 · Paroxysmal hypersynchronous discharges in the neuropil calcium signal are observ, H2.P4.1 · 8 of 12 Kcnc1-A421V/+ mice exhibit convulsive spontaneous seizures on video-EEG , H2.P2 · Awake Kcnc1-A421V/+ mice should show two-photon signatures of cortical disinhibi, H2.P4 · Kcnc1-A421V/+ mice should show spontaneous convulsive seizures, SUDEP events, an
[8]
direct map → H2.P3.2 · PV-IN-mediated inhibitory synaptic transmission is not significantly altered in , H2.P3.1 · In adult (P32–42) Kcnc1-A421V/+ mice, PV-IN-mediated uIPSC magnitude is signific, S1 · Inhibitory neurotransmission shows no significant alterations at juvenile stage , H2.P3 · PV-IN→excitatory unitary inhibitory transmission should be intact at juvenile st
[9]
synthesis across claims → H1 · KCNC1-A421V is a Kv3.1 loss-of-function variant whose primary lesion is impaired, H3 · Kv3.1 loss-of-function should selectively impair fast-spiking PV-INs that depend, H2 · Cell-autonomous Kv3.1 LOF in PV-INs is sufficient to drive the KCNC1 DEE phenoty, S1 · Inhibitory neurotransmission shows no significant alterations at juvenile stage
Claims in the graph not surfaced in the abstract
  • D1 a421v-weight-reduced-milestones-normal fig1—figure supplement 2B, 2C, 2D, 2E
    Kcnc1-A421V/+ mice show significantly reduced body and brain weight relative to WT littermates, but display no detectable abnormalities in gross developmental milestones (fur appearance, eye opening, ear canal opening, incisor eruption, motor benchmarks) assessed at P5–15.
  • H3.P2.1 layer-v-pv-ins-subtle-impairment fig4—figure supplement 2
    Layer V neocortical PV-INs from juvenile (P16–21) Kcnc1-A421V/+ mice show more subtle abnormalities than layer II-IV PV-INs: AP frequency reduction is confined to the largest current injection magnitudes (***p<0.001 for genotype × current injection interaction), consistent with the higher relative expression of Kv3.2 vs Kv3.1 in deeper cortical layers.
  • H3.P2.2 rtn-neurons-impaired-excitability fig4—figure supplement 3
    Parvalbumin-positive reticular thalamic nucleus (RTN) neurons from juvenile (P16–21) Kcnc1-A421V/+ mice generate fewer rebound APs in response to hyperpolarizing current and show attenuated frequency-current relationship (*p=0.0109), with significantly reduced AP downstroke velocity (*p=0.034) but preservation of other membrane properties.
  • H3.P2 prediction-impairment-grades-with-kv31-dependence prediction
    Phenotype magnitude across PV-IN populations should grade with Kv3.1 dependence: superficial cortical (largest), layer V (mildest), RTN (intermediate, distinctive rebound-firing pattern).

How it is defined

A model answers this layer, so the prompt is the layer. It is reproduced below from the committed file, and it is a declared input — editing it makes every run that used it stale.

extract/prompts/abstract-map.mdthe prompt it runs undernot in the repository

The declaration names this path and the repository does not have it. An input that does not exist hashes to nothing, so it cannot make a run stale — the layer is declared to depend on something it is not in fact tracking.

Artifacts

Versions

From the run ledger. There is no changelog beside it to keep in step.

  1. v1 · 2026-04-20 · unrecorded backfilled from the artifact

    backfilled from the artifact on disk

This layer across the corpus

Across the corpus

9 run not observed · 1 stale·a paper links to its own cell, where this layer's output for it is rendered

PaperStateVersionLast runOutputCell
A three-dimensional immunofluorescence atlas of the …backfilled from the artifact on diskrun not observedv12026-04-20artiushin-2026-spider-atlas.jsonjson
Distinct representational properties of cues and con…backfilled from the artifact on diskrun not observedv12026-04-20bouyeure-2026-fear-rsa.jsonjson
Computational modelling identifies key determinants …backfilled from the artifact on diskrun not observedv12026-04-20ejdrup-2026-dopamine.jsonjson
Contributions of insula and superior temporal sulcus…re-run for the current treestalev52026-09-12gadeke-2026-guilt-insula.jsonjson
Spatially targeted inhibitory rhythms differentially…backfilled from the artifact on diskrun not observedv12026-04-19headley-2026-inhibitory-rhythms.jsonjson
Feedback of peripheral saccade targets to early fove…backfilled from the artifact on diskrun not observedv12026-04-19kammer-2026-foveal-feedback.jsonjson
iGABASnFR2 is an improved genetically encoded protei…backfilled from the artifact on diskrun not observedv12026-04-20kolb-2026-igabasnfr2.jsonjson
A deep learning pipeline for mapping in situ network…backfilled from the artifact on diskrun not observedv12026-04-20rozak-2026-neurovascular-dl.jsonjson
Self-association enhances early attentional selectio…backfilled from the artifact on diskrun not observedv12026-04-20scheller-2026-self-prioritization.jsonjson
Impaired excitability of fast-spiking neurons in a n…backfilled from the artifact on diskrun not observedv12026-04-20wengert-2026-kcnc1.jsonjson

Inputs and outputs

Reads, besides its dependencies
Produces
  • site/src/data/abstract-mapping/{paper}.json

One per paper — the table above links each one that exists.

Views
  • document — rendered above, from the artifact itself
  • comparison — on the cell page, two versions aligned by the matcher, wherever the ledger holds more than one

Running it

The command comes from the declaration, so this text and what actually runs cannot diverge. pipeline.py run also runs the unmet dependencies first.

python3 scripts/pipeline.py run <paper> abstract-map

Underneath, that runs cd extract && python3 -m claim_graphs.cli abstract-map --paper {paper}.